Purification of rat liver S-adenosyl-L-methionine decarboxylase.
نویسنده
چکیده
The amount of S-adenosyl-l-methionine decarboxylase present in rat liver was enhanced 17-fold by administration of methylglyoxal bis(guanylhydrazone),* a specific inhibitor of the enzyme. The enzyme was purified 1400-fold in 50% yield from such liver extracts by chromatography on columns of the inhibitor bound to Sepharose. The purified enzyme had no spermidine synthetase activity.
منابع مشابه
Indirect evidence for a strict negative control of S-adenosyl-L-methionine decarboxylase by spermidine in rat hepatoma cells.
1. Direct or indirect inhibitors of l-ornithine decarboxylase (EC 4.1.1.17), structurally related or unrelated to l-ornithine, including dl-alpha-difluoromethylornithine, alpha-methylornithine and 1,3-diaminopropane, used alone or in combination, decreased polyamine concentrations in rat hepatoma tissue culture (HTC) cells and increased S-adenosyl-l-methionine decarboxylase activity (EC 4.1.1.5...
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The enzymatic activities for the synthesis of phosphatidylcholine from phosphatidylethanolamine and Sadenosyl-L-methionine have been solubilized from rat liver microsomes by ultrasonic disruption in the presence of 0.2% Triton X-100. Significant co-purification of the activities was achieved by chromatography of the Triton extract on octyl-Sepharose CL-4B and, subsequently, on Sepharose 6B. Thr...
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The enzymatic activities for the synthesis of phosphatidylcholine from phosphatidylethanolamine and Sadenosyl-L-methionine have been solubilized from rat liver microsomes by ultrasonic disruption in the presence of 0.2% Triton X-100. Significant co-purification of the activities was achieved by chromatography of the Triton extract on octyl-Sepharose CL-4B and, subsequently, on Sepharose 6B. Thr...
متن کاملInhibition of spermidine formation in rat liver and kidney by methylglyoxal bis(guanylhydrazone).
The effect of methylglyoxal bis(guanylhydrazone), a substance known to inhibit putrescine-dependent S-adenosyl-l-methionine decarboxylase, on polyamine metabolism in liver and kidney was investigated. Almost complete inhibition of the incorporation of putrescine into spermidine was obtained up to 8h after administration of 80mg of methylglyoxal bis(guanylhydrazone)/kg body wt. by intraperitonea...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 141 2 شماره
صفحات -
تاریخ انتشار 1974